Re: Embryo Rescue

What is the cause of the failure to develop endosperm in these
intergeneric hybrids?

Message: 1
Date: Thu, 1 Apr 2021 17:00:51 +0000 (UTC)
From: Robert Lauf <boblauf@att.net>
To: Pacific Bulb Society <pbs@lists.pacificbulbsociety.net>
Subject: [pbs] Embryo Rescue
Message-ID: <176250100.3124430.1617296451883@mail.yahoo.com>
Content-Type: text/plain; charset="utf-8"

I recently made an intergeneric cross involving Hippeastrum and got a
bunch of seeds that had little or no endosperm but otherwise looked ripe.?
The seeds were surface-sterilized in hydrogen peroxide and placed in
sterile culture medium.? A photo of the first baby is attached.? She says
it looks like more are going to develop but they aren't as far along.

A useful method that makes some wide crosses possible and is practically
identical to the familiar techniques used for germinating orchid seeds.?
With care, you can even do it at home in a little home-brew glovebox, altho
this was done in a professional lab facility (laminar flow hood, etc.)?
Yes, it's nice to have friends in high (or at least clean) places!

Bob? Zone 7? after 7" of rain in the last week or so, now there's a
threatened freeze tonight.? Spring in East Tenn...
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