> > Re: Embryo Rescue > What is the cause of the failure to develop endosperm in these > intergeneric hybrids? > > Message: 1 > Date: Thu, 1 Apr 2021 17:00:51 +0000 (UTC) > From: Robert Lauf <boblauf@att.net> > To: Pacific Bulb Society <pbs@lists.pacificbulbsociety.net> > Subject: [pbs] Embryo Rescue > Message-ID: <176250100.3124430.1617296451883@mail.yahoo.com> > Content-Type: text/plain; charset="utf-8" > > I recently made an intergeneric cross involving Hippeastrum and got a > bunch of seeds that had little or no endosperm but otherwise looked ripe.? > The seeds were surface-sterilized in hydrogen peroxide and placed in > sterile culture medium.? A photo of the first baby is attached.? She says > it looks like more are going to develop but they aren't as far along. > > A useful method that makes some wide crosses possible and is practically > identical to the familiar techniques used for germinating orchid seeds.? > With care, you can even do it at home in a little home-brew glovebox, altho > this was done in a professional lab facility (laminar flow hood, etc.)? > Yes, it's nice to have friends in high (or at least clean) places! > > Bob? Zone 7? after 7" of rain in the last week or so, now there's a > threatened freeze tonight.? Spring in East Tenn... > -------------- next part -------------- > A non-text attachment was scrubbed... > Name: Embryo rescue.jpg > Type: image/jpeg > Size: 97158 bytes > Desc: not available > URL: < > http://lists.pacificbulbsociety.net/pipermail/pbs/… > > > > ------------------------------ > > ********************************** > _______________________________________________ pbs mailing list pbs@lists.pacificbulbsociety.net http://lists.pacificbulbsociety.net/cgi-bin/… Unsubscribe: <mailto:pbs-unsubscribe@lists.pacificbulbsociety.net>