We always provide some kind of humidity tent for anything we take out of culture unless they are dormant and underground (dormant lilies and trillium). Otherwise they turn into crispy critters pretty quickly. So we’re on top of that at least. I decided to plant the Fritillaria eduardii in straight pumice and put them under my grow lights inside a plastic propagator. If they don’t kick the bucket quickly I’ll slowly decrease the humidity. Several of them have nice roots for what it’s worth. A few also had some leaves; I cut those off so they wouldn’t rot. I can’t include an attachment showing their roots here but if anyone is interested I can send it privately. Does anyone want to argue I should put them outside in my greenhouse for a winter chill? So what have “the Tims” tissue cultured or tried to culture? Emma and I have done lilies, trilliums, erthryoniums and jankea (not a geophyte) with varying levels of success. We’re also struggling to get jankea out of culture. Jan Jeddeloh, Portland, Oregon, soggy zone 8 > On Dec 11, 2016, at 7:22 AM, Tim Eck <teck11@embarqmail.com> wrote: > > I have very limited experience with tissue culture and similar procedures > like orchid and fern (spore) propagation and nut-grafting. > Failure modes I have experienced or read about include: > > Terrestrials often need charcoal in the agar to fully differentiate roots. > You may need some sort of humidity control or tent to wean them from near > 100% in vitrio. (Transplanting staghorn ferns at .5" size was done in the > bathroom with the hot shower providing the humidity. Otherwise they would > be dead in seconds.) > Expect it to take a few years to achieve the vigor of a first year seedling. > > Tim Eck > > >> -----Original Message----- >> From: pbs [mailto:pbs-bounces@lists.ibiblio.org] On Behalf Of Jane McGary >> Sent: Saturday, December 10, 2016 1:02 PM >> To: Pacific Bulb Society >> Subject: [pbs] Fritillaria from tissue culture >> >> Two friends who are also PBS members, Jan Jeddeloh and Emma Elliott, >> raised some Fritillaria eduardii in tissue culture using tissue from the > ovary of >> a bud on my only plant of this species. (They're having success with > Lilium >> species.) Jan would like to wean the young plants out of the agar medium >> now, but previous attempts (including one by me) have failed. F. eduardii > is a >> member of the Imperiales section, which includes the familiar garden > species >> Fritillaria imperialis and Fritillaria raddeana. Does anyone on this forum > have >> experience growing these by tissue culture, with success at the crucial > point >> of moving from sterile medium to growing in an ordinary pot? Can you offer >> advice, please? >> >> Jane McGary >> >> Portland, Oregon >> >> >> _______________________________________________ >> pbs mailing list >> pbs@lists.ibiblio.org >> http://pacificbulbsociety.org/list.php >> http://pacificbulbsociety.org/pbswiki/ > > _______________________________________________ > pbs mailing list > pbs@lists.ibiblio.org > http://pacificbulbsociety.org/list.php > http://pacificbulbsociety.org/pbswiki/ _______________________________________________ pbs mailing list pbs@lists.ibiblio.org http://pacificbulbsociety.org/list.php http://pacificbulbsociety.org/pbswiki/