Fritillaria from tissue culture
Tim Eck (Sun, 11 Dec 2016 07:22:04 PST)

I have very limited experience with tissue culture and similar procedures
like orchid and fern (spore) propagation and nut-grafting.
Failure modes I have experienced or read about include:

Terrestrials often need charcoal in the agar to fully differentiate roots.
You may need some sort of humidity control or tent to wean them from near
100% in vitrio. (Transplanting staghorn ferns at .5" size was done in the
bathroom with the hot shower providing the humidity. Otherwise they would
be dead in seconds.)
Expect it to take a few years to achieve the vigor of a first year seedling.

Tim Eck

-----Original Message-----
From: pbs [mailto:pbs-bounces@lists.ibiblio.org] On Behalf Of Jane McGary
Sent: Saturday, December 10, 2016 1:02 PM
To: Pacific Bulb Society
Subject: [pbs] Fritillaria from tissue culture

Two friends who are also PBS members, Jan Jeddeloh and Emma Elliott,
raised some Fritillaria eduardii in tissue culture using tissue from the

ovary of

a bud on my only plant of this species. (They're having success with

Lilium

species.) Jan would like to wean the young plants out of the agar medium
now, but previous attempts (including one by me) have failed. F. eduardii

is a

member of the Imperiales section, which includes the familiar garden

species

Fritillaria imperialis and Fritillaria raddeana. Does anyone on this forum

have

experience growing these by tissue culture, with success at the crucial

point

of moving from sterile medium to growing in an ordinary pot? Can you offer
advice, please?

Jane McGary

Portland, Oregon

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