Bob, I don't have info on micropropagation, which is what you want, but I have done both the 'sliver' (see below) and sectioning a third of the bulb, both to preserve the original bulb. It worked for me on common hybrid Hippeastrum- unsure about species bulbs. I'm not sure I would dare cut a bulb I really cared about! I've tried twin-scaling, and it needs more controlled conditions than I was willing to provide for success. The 'sliver' method is detailed on the PBS list archive from dec 2007. I quoted it below, in case the link gets stripped by the listserv. The post is by Dr. James W. Waddick, and the sliver technique he is quoting is from Bill Warren. Amaryllis Basics II - Slivering (pacificbulbsociety.org) <https://pacificbulbsociety.org/pbslist/…> https://pacificbulbsociety.org/pbslist/… "2.Propagation: Slivering Technique by Bill Warren of the Amaryllis Study Group "This method keeps the "mother" bulb alive and blooming while you wait for mature offspring (clones). I developed it from existing vegetative cloning techniques when I did one and had a crop failure and could get no more of that variety. "I developed a great technique I call 'Slivering' where you take a sliver from the bottom corner of the whole bulb right to the center You can take up to 4 Slivers without harming the original bulb. Keep them thin (width of one root) and make sure they each have some of the bulb scales, root crown, a single whole root or 2, and the stem/base in the center. It is like making your own offsets. See the link for more details. Another reference to this technique, explained a little differently, is in the link below, and may have originated from an IBS message board. Warren: Easy Propagaation (x10.mx) <http://bulbnrose.x10.mx/Amaryllis/IBS/…> http://bulbnrose.x10.mx/Amaryllis/IBS/… I've had this article (below) bookmarked for some time as well. I followed the recommendation to section bulbs into 8ths and have done that several times with great success- again with the cheap commercial hybrid hippeastrums. Again, the link may be stripped out- so copy and paste it. (PDF) Vegetative Propagation of Amaryllis (Hippeastrum × johnsonii ) by Different Cutting Methods (researchgate.net) <https://researchgate.net/publication/…> https://researchgate.net/publication/… It's Christmas, and the stores are full of test Amaryllis to perfect your cuttage or tissue culture skills. Sorry I don't have what you really wanted. Ken On Fri, Dec 1, 2023 at 7:41 AM Robert Lauf via pbs < pbs@lists.pacificbulbsociety.net> wrote: > Does anyone have a recommendation for vegetative propagation of > Hippeastrum? In this case it is important to have a method that doesn't > risk losing the entire plant, so I prefer not to dice up the whole bulb and > hope the chips survive. I don't think leaf props work as easily as in > Eucomis, if at all, but ideally some sort of tissue culture using leaves > would be preferred. I'm prepared to do sterile culture if someone has any > experience with the medium and protocols that work for Hipps. I plan to > dig around in the literature but if someone is already experienced in this, > I'm all ears. Thanks! > Bob Lauf Zone 7 40s and rain today > _______________________________________________ > pbs mailing list > pbs@lists.pacificbulbsociety.net > https://lists.pacificbulbsociety.net/cgi-bin/… > Unsubscribe: <mailto:pbs-unsubscribe@lists.pacificbulbsociety.net> > PBS Forum latest: > https://pacificbulbsociety.org/pbsforum/index.php/… > _______________________________________________ pbs mailing list pbs@lists.pacificbulbsociety.net https://lists.pacificbulbsociety.net/cgi-bin/… Unsubscribe: <mailto:pbs-unsubscribe@lists.pacificbulbsociety.net> PBS Forum https://…