Hippeastrum produces a red phenolic compound which will color tissue culture media and sometimes appears on twin scales (or other injured tissue). Alan Meerow ----- Original Message ----- From: <Bonsaigai37@aol.com> To: <pbs@lists.ibiblio.org> Sent: Monday, December 08, 2003 8:34 PM Subject: [pbs] twin scaling quandry > Hello Group, > > I have a question about twin scaling Hippeastrum. After following a combined > procedure by RHS and IBS, using Cleary's 3336F on the scales, in sterilized > perlite, I'm noticing an even pink coloration on the scales. I think, hmmm, > damnable Stagonospora... yet I used Bonomyl (Benlate sub) for soaking the bulb > prior to storage, Bleach (1:10) prior to cuttage, and 3336F for the final soak, > alcohol rinsed surfaces and sterile razors. Do Hippeastrum show red or pink > as a form or oxidation to damaged cells or is my culture simply contaminated? > Any remedies? > > OH... I just joined the list. I'm Michael Loos, Cleveland, > Ohio-ly-mackerel-it's-cold. I grow all sorts of geophytes including succulent caudiciforms, > fat - in and out of the ground. I've lots and ones and twos (3's-4's) of many > species, but heavy on the genera of Hippeastrum and Euphorbia. Presently, I'm > building my species list of Hippeastrum. (Anyone with species Hipps for sale > or trade, please feel free to contact me.) > > Thanks for the information, > Grow Well, > Michael Loos > Honest Earth Consultation > _______________________________________________ > pbs mailing list > pbs@lists.ibiblio.org > http://www.pacificbulbsociety.org/list.php > >