twin scaling quandry
Nolo Contendre (Tue, 09 Dec 2003 02:40:21 PST)
Hippeastrum produces a red phenolic compound which will color tissue culture
media and sometimes appears on twin scales (or other injured tissue).
Alan Meerow
----- Original Message -----
From: <Bonsaigai37@aol.com>
To: <pbs@lists.ibiblio.org>
Sent: Monday, December 08, 2003 8:34 PM
Subject: [pbs] twin scaling quandry
Hello Group,
I have a question about twin scaling Hippeastrum. After following a
combined
procedure by RHS and IBS, using Cleary's 3336F on the scales, in
sterilized
perlite, I'm noticing an even pink coloration on the scales. I think,
hmmm,
damnable Stagonospora... yet I used Bonomyl (Benlate sub) for soaking the
bulb
prior to storage, Bleach (1:10) prior to cuttage, and 3336F for the final
soak,
alcohol rinsed surfaces and sterile razors. Do Hippeastrum show red or
pink
as a form or oxidation to damaged cells or is my culture simply
contaminated?
Any remedies?
OH... I just joined the list. I'm Michael Loos, Cleveland,
Ohio-ly-mackerel-it's-cold. I grow all sorts of geophytes including
succulent caudiciforms,
fat - in and out of the ground. I've lots and ones and twos (3's-4's) of
many
species, but heavy on the genera of Hippeastrum and Euphorbia. Presently,
I'm
building my species list of Hippeastrum. (Anyone with species Hipps for
sale
or trade, please feel free to contact me.)
Thanks for the information,
Grow Well,
Michael Loos
Honest Earth Consultation
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