twin scaling quandry
Nolo Contendre (Tue, 09 Dec 2003 02:40:21 PST)

Hippeastrum produces a red phenolic compound which will color tissue culture
media and sometimes appears on twin scales (or other injured tissue).

Alan Meerow
----- Original Message -----
From: <Bonsaigai37@aol.com>
To: <pbs@lists.ibiblio.org>
Sent: Monday, December 08, 2003 8:34 PM
Subject: [pbs] twin scaling quandry

Hello Group,

I have a question about twin scaling Hippeastrum. After following a

combined

procedure by RHS and IBS, using Cleary's 3336F on the scales, in

sterilized

perlite, I'm noticing an even pink coloration on the scales. I think,

hmmm,

damnable Stagonospora... yet I used Bonomyl (Benlate sub) for soaking the

bulb

prior to storage, Bleach (1:10) prior to cuttage, and 3336F for the final

soak,

alcohol rinsed surfaces and sterile razors. Do Hippeastrum show red or

pink

as a form or oxidation to damaged cells or is my culture simply

contaminated?

Any remedies?

OH... I just joined the list. I'm Michael Loos, Cleveland,
Ohio-ly-mackerel-it's-cold. I grow all sorts of geophytes including

succulent caudiciforms,

fat - in and out of the ground. I've lots and ones and twos (3's-4's) of

many

species, but heavy on the genera of Hippeastrum and Euphorbia. Presently,

I'm

building my species list of Hippeastrum. (Anyone with species Hipps for

sale

or trade, please feel free to contact me.)

Thanks for the information,
Grow Well,
Michael Loos
Honest Earth Consultation
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