Helicon focus fully automates the process and creates stunning photos, assuming you have a DSLR camera. I did a presentation including some work done with Helicon Focus at the International Aroid Conference in Hanoi last December and got some botanists very excited. Also creates a 3d image of the subject. Greg Ruckert, Nairne, South Australia ----- Original Message ----- From: "David Ehrlich" <idavide@sbcglobal.net> To: "Pacific Bulb Society" <pbs@lists.ibiblio.org> Sent: Sunday, February 09, 2014 2:26 PM Subject: Re: [pbs] Best dissecting microscopes for botanical subjects? I had heard about, though I don't know where to find more information on it, a camera + computer setup where the camera took a series of pictures with gradually changing focus settings, and the pictures were then combined by the computer choosing only the in-focus portions to create the composite. This would be extremely useful in creating substantial depth of field for close-ups, and macro photography generally. If anyone knows anything about this equipment, I'd be very interested. >________________________________ > From: Tim Eck <teck11@embarqmail.com> >To: 'Pacific Bulb Society' <pbs@lists.ibiblio.org> >Sent: Saturday, February 8, 2014 12:06 PM >Subject: Re: [pbs] Best dissecting microscopes for botanical subjects? > > >It may be worth mentioning if anyone here is a software guru or twelve year >old, there are some really cool things you can do with photomicrographs >nowadays to remove all blurring from limited depth of field. It involves >taking several images where the only thing you change is the focal distance >and then create a composite saving only the in-focus parts of each image. >This is surprisingly simple for digital images since a sharp sub-image or >neighborhood is defined by high local variation in brightness as measured >by >standard deviation or range. > > > >_______________________________________________ >pbs mailing list >pbs@lists.ibiblio.org >http://pacificbulbsociety.org/list.php >http://pacificbulbsociety.org/pbswiki/ > > > _______________________________________________ pbs mailing list pbs@lists.ibiblio.org http://pacificbulbsociety.org/list.php http://pacificbulbsociety.org/pbswiki/