>Dear All; > I am looking for help. advise and especially experience with >tissue culturing of bulbs*. > Can you recommend a reliable TC lab? Name/web site/ etc > > Have you had experience trying to get a bulb of your own into >TC? What are the pitfalls? > > Appreciate any first hand comments. Jim W. > >* in the broadest sense including both bulbous and rhizomateous iris Jim: If you mean commercial work I have been in contact with a lab in India (very infrequent e-mails so far and that's not encouraging) that seem to have an excellent program for callas and a few other species. I've spent months trying to clean up alstroemeria rhizomes to get them to increase in vitro (potentially much more rapid). The literature (viz. Mark Bridgen of U Conn and some Indian labs, and Googling), suggests that it should be relatively easy to get them free of bacteria and fungi, but I had zero success, even working with TC experts at Davis who succeeded with other species. I never understood what we were doing wrong nor how we differed in methodology from that in the publications we followed. Roy