I am curious to know what goal you have in mind by using tissue culture. Propagation? Virus clean-up? The reason I ask is that there are easier methods of propagation that you can use at home where you don't have to consider costs and loss of valuable propagules due to media contamination. You can accomplish quite a bit with premium rooting hormone, the proper rooting conditions and a knife. The project that was the subject of my Master's degree thesis was grounded in tissue culture. I worked with the genetic component of somatic embryogenesis in roses. Tissue culture can get very expensive and be very frustrating. Please clarify your goals a bit if you have a chance. Sincerely, Millie Burrell >>> jwaddick@kc.rr.com 07/20/05 9:02 AM >>> Dear All; I am looking for help. advise and especially experience with tissue culturing of bulbs*. Can you recommend a reliable TC lab? Name/web site/ etc Have you had experience trying to get a bulb of your own into TC? What are the pitfalls? Appreciate any first hand comments. Jim W. * in the broadest sense including both bulbous and rhizomateous iris -- Dr. James W. Waddick 8871 NW Brostrom Rd. Kansas City Missouri 64152-2711 USA Ph. 816-746-1949 Zone 5 Record low -23F Summer 100F + _______________________________________________ pbs mailing list pbs@lists.ibiblio.org http://www.pacificbulbsociety.org/list.php