I have very limited experience with tissue culture and similar procedures like orchid and fern (spore) propagation and nut-grafting. Failure modes I have experienced or read about include: Terrestrials often need charcoal in the agar to fully differentiate roots. You may need some sort of humidity control or tent to wean them from near 100% in vitrio. (Transplanting staghorn ferns at .5" size was done in the bathroom with the hot shower providing the humidity. Otherwise they would be dead in seconds.) Expect it to take a few years to achieve the vigor of a first year seedling. Tim Eck > -----Original Message----- > From: pbs [mailto:pbs-bounces@lists.ibiblio.org] On Behalf Of Jane McGary > Sent: Saturday, December 10, 2016 1:02 PM > To: Pacific Bulb Society > Subject: [pbs] Fritillaria from tissue culture > > Two friends who are also PBS members, Jan Jeddeloh and Emma Elliott, > raised some Fritillaria eduardii in tissue culture using tissue from the ovary of > a bud on my only plant of this species. (They're having success with Lilium > species.) Jan would like to wean the young plants out of the agar medium > now, but previous attempts (including one by me) have failed. F. eduardii is a > member of the Imperiales section, which includes the familiar garden species > Fritillaria imperialis and Fritillaria raddeana. Does anyone on this forum have > experience growing these by tissue culture, with success at the crucial point > of moving from sterile medium to growing in an ordinary pot? Can you offer > advice, please? > > Jane McGary > > Portland, Oregon > > > _______________________________________________ > pbs mailing list > pbs@lists.ibiblio.org > http://pacificbulbsociety.org/list.php > http://pacificbulbsociety.org/pbswiki/